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Assay Detail

CHEMBL753633

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]- diprenorphine from mu-W318A-receptor expressed in HEK 293 cells
5
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL270)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Investigation of the selectivity of oxymorphone- and naltrexone-derived ligands via site-directed mutagenesis of opioid receptors: exploring the "address" recognition locus.
Metzger TG, Paterlini MG, Ferguson DM, Portoghese PS.
J Med Chem (2001) 44 : 857 -862
PubMed 11300867 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 9.08 9.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL573214 NORBINALTORPHIMINE 2 9.66
CHEMBL485832 GUANIDINONALTRINDOLE 1 9.30
CHEMBL19019 NALTREXONE 4.0 1 8.60
CHEMBL399708 SPIROINDANYLOXYMORPHONE 1 8.41

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL573214 NORBINALTORPHIMINE Ki = 0.22 nM 9.66
CHEMBL573214 NORBINALTORPHIMINE Ki = 0.39 nM 9.41
CHEMBL485832 GUANIDINONALTRINDOLE Ki = 0.5 nM 9.30
CHEMBL19019 NALTREXONE Ki = 2.5 nM 8.60
CHEMBL399708 SPIROINDANYLOXYMORPHONE Ki = 3.9 nM 8.41