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Assay Detail

CHEMBL754165

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibitory activity against P-glycoprotein expressed in murine mammary carcinoma (EMT6/AR1.0 cell line) in accumulation assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

ATP-dependent translocase ABCB1 (CHEMBL2573)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Design and synthesis of new templates derived from pyrrolopyrimidine as selective multidrug-resistance-associated protein inhibitors in multidrug resistance.
Wang S, Wan NC, Harrison J, Miller W, Chuckowree I, Sohal S, Hancox TC, Baker S, Folkes A, Wilson F, Thompson D, Cocks S, Farmer H, Boyce A, Freathy C, Broadbridge J, Scott J, Depledge P, Faint R, Mistry P, Charlton P.
J Med Chem (2004) 47 : 1339 -1350
PubMed 14998324 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 4.91 5.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL27128 1 5.48
CHEMBL29743 1 5.21
CHEMBL285343 1 5.16
CHEMBL29631 1 5.16
CHEMBL431503 1 5.08
CHEMBL26081 1 5.08
CHEMBL284024 1 5.08
CHEMBL25867 1 5.04
CHEMBL27536 1 5.04
CHEMBL30052 1 5.00
CHEMBL26039 1 4.98
CHEMBL430925 1 4.94
CHEMBL283693 1 4.84
CHEMBL28584 1 4.76
CHEMBL282156 1 4.74
CHEMBL26369 1 4.67
CHEMBL430724 1 4.65
CHEMBL281235 1 4.61
CHEMBL283438 1 4.55
CHEMBL25972 1 4.55
CHEMBL27257 1 4.40
CHEMBL287181 1 -
CHEMBL281284 1 -
CHEMBL281743 1 -
CHEMBL27191 1 -
CHEMBL286963 1 -
CHEMBL283255 1 -
CHEMBL439727 1 -
CHEMBL26678 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL27128 IC50 = 3310.0 nM 5.48
CHEMBL29743 IC50 = 6140.0 nM 5.21
CHEMBL285343 IC50 = 6940.0 nM 5.16
CHEMBL29631 IC50 = 6940.0 nM 5.16
CHEMBL431503 IC50 = 8300.0 nM 5.08
CHEMBL26081 IC50 = 8400.0 nM 5.08
CHEMBL284024 IC50 = 8220.0 nM 5.08
CHEMBL25867 IC50 = 9230.0 nM 5.04
CHEMBL27536 IC50 = 9200.0 nM 5.04
CHEMBL30052 IC50 = 10000.0 nM 5.00
CHEMBL26039 IC50 = 10500.0 nM 4.98
CHEMBL430925 IC50 = 11600.0 nM 4.94
CHEMBL283693 IC50 = 14300.0 nM 4.84
CHEMBL28584 IC50 = 17200.0 nM 4.76
CHEMBL282156 IC50 = 18040.0 nM 4.74
CHEMBL26369 IC50 = 21600.0 nM 4.67
CHEMBL430724 IC50 = 22500.0 nM 4.65
CHEMBL281235 IC50 = 24550.0 nM 4.61
CHEMBL283438 IC50 = 28300.0 nM 4.55
CHEMBL25972 IC50 = 27860.0 nM 4.55
CHEMBL27257 IC50 = 40000.0 nM 4.40
CHEMBL287181 IC50 > 40000.0 nM -
CHEMBL281284 IC50 > 40000.0 nM -
CHEMBL281743 IC50 > 40000.0 nM -
CHEMBL27191 IC50 > 40000.0 nM -
CHEMBL286963 IC50 > 40000.0 nM -
CHEMBL283255 IC50 > 40000.0 nM -
CHEMBL439727 IC50 > 40000.0 nM -
CHEMBL26678 IC50 > 40000.0 nM -