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Assay Detail

CHEMBL754645

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Tested to inhibit the conversion of [3H]L-arginine to [3H]L-citrulline catalyzed by inducible NOS from mouse macrophage J774 cells
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type J774
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Nitric oxide synthase, inducible (CHEMBL3464)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

1,2-diaminobenzimidazoles : Selective inhibitors of nitric oxide synthase derived from aminoguanidine
Hamley P, Tinker AC
Bioorg Med Chem Lett (1995) 5 : 1573 -1576
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.75 5.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL225304 PIMAGEDINE 2.0 1 5.05
CHEMBL247378 7-NITROINDAZOLE 1 4.70
CHEMBL41060 1 4.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL225304 PIMAGEDINE IC50 = 9000.0 nM 5.05
CHEMBL247378 7-NITROINDAZOLE IC50 = 20000.0 nM 4.70
CHEMBL41060 IC50 = 31000.0 nM 4.51