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Assay Detail

CHEMBL754825

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity towards Opioid receptor kappa 1 using [3H]diprenorphine as radioligand in guinea pig cerebellar membrane in absence of 120 mM NaCl + 50 uM Gpp(NH)p
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Tissue Cerebellum
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kappa-type opioid receptor (CHEMBL3952)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Synthesis and opioid activity of [D-Pro10]dynorphin A-(1-11) analogues with N-terminal alkyl substitution.
Choi H, Murray TF, DeLander GE, Schmidt WK, Aldrich JV.
J Med Chem (1997) 40 : 2733 -2739
PubMed 9276018 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 8.47 9.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL384584 1 9.59
CHEMBL442323 1 9.39
CHEMBL406338 1 8.64
CHEMBL406893 1 8.48
CHEMBL268818 1 8.13
CHEMBL266782 1 7.67
CHEMBL267215 1 7.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL384584 IC50 = 0.26 nM 9.59
CHEMBL442323 IC50 = 0.41 nM 9.39
CHEMBL406338 IC50 = 2.3 nM 8.64
CHEMBL406893 IC50 = 3.31 nM 8.48
CHEMBL268818 IC50 = 7.32 nM 8.13
CHEMBL266782 IC50 = 21.3 nM 7.67
CHEMBL267215 IC50 = 43.2 nM 7.37