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Assay Detail

CHEMBL755105

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of neutral endopeptidase activity in rabbit kidney with 20 nM [3H]D-Ala2-Leu-enkephalin as substrate
16
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Oryctolagus cuniculus
Confidence 9 — Direct single protein target
Curated By Expert

Target

Neprilysin (CHEMBL3768)
Type SINGLE PROTEIN
Organism Oryctolagus cuniculus

Publication

New kelatorphan-related inhibitors of enkephalin metabolism: improved antinociceptive properties.
Xie J, Soleilhac JM, Schmidt C, Peyroux J, Roques BP, Fournié-Zaluski MC.
J Med Chem (1989) 32 : 1497 -1503
PubMed 2738884 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 8.24 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL16779 1 8.85
CHEMBL36094 2 8.77
CHEMBL290337 1 8.74
CHEMBL37921 1 8.64
CHEMBL440104 1 8.62
CHEMBL37418 1 8.60
CHEMBL2114407 1 8.54
CHEMBL2115347 1 8.52
CHEMBL2114408 1 8.52
CHEMBL2115348 1 8.12
CHEMBL285617 2 7.89
CHEMBL417878 1 7.56
CHEMBL36841 1 7.29
CHEMBL290366 1 6.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL16779 IC50 = 1.4 nM 8.85
CHEMBL36094 IC50 = 1.7 nM 8.77
CHEMBL290337 IC50 = 1.8 nM 8.74
CHEMBL36094 IC50 = 2.0 nM 8.70
CHEMBL37921 IC50 = 2.3 nM 8.64
CHEMBL440104 IC50 = 2.4 nM 8.62
CHEMBL37418 IC50 = 2.5 nM 8.60
CHEMBL2114407 IC50 = 2.9 nM 8.54
CHEMBL2115347 IC50 = 3.0 nM 8.52
CHEMBL2114408 IC50 = 3.0 nM 8.52
CHEMBL2115348 IC50 = 7.6 nM 8.12
CHEMBL285617 IC50 = 13.0 nM 7.89
CHEMBL285617 IC50 = 26.5 nM 7.58
CHEMBL417878 IC50 = 27.5 nM 7.56
CHEMBL36841 IC50 = 51.5 nM 7.29
CHEMBL290366 IC50 = 150.0 nM 6.82