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Assay Detail

CHEMBL755490

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]diprenorphine from opioid receptor kappa 1 expressed in HEK 293 cells
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Kappa-type opioid receptor (CHEMBL3614)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Investigation of the selectivity of oxymorphone- and naltrexone-derived ligands via site-directed mutagenesis of opioid receptors: exploring the "address" recognition locus.
Metzger TG, Paterlini MG, Ferguson DM, Portoghese PS.
J Med Chem (2001) 44 : 857 -862
PubMed 11300867 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 7.74 9.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL485832 GUANIDINONALTRINDOLE 1 9.74
CHEMBL19019 NALTREXONE 4.0 1 8.38
CHEMBL567175 NALTRINDOLE 1 7.59
CHEMBL963 OXYMORPHONE 4.0 1 6.68
CHEMBL399708 SPIROINDANYLOXYMORPHONE 1 6.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL485832 GUANIDINONALTRINDOLE Ki = 0.18 nM 9.74
CHEMBL19019 NALTREXONE Ki = 4.2 nM 8.38
CHEMBL567175 NALTRINDOLE Ki = 25.5 nM 7.59
CHEMBL963 OXYMORPHONE Ki = 208.0 nM 6.68
CHEMBL399708 SPIROINDANYLOXYMORPHONE Ki = 488.0 nM 6.31