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Assay Detail

CHEMBL755496

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]dynorphin A from Opioid receptor kappa 1 of rat brain membranes (DAMGO/DADLE quenching of mu/delta receptor binding)
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Expert

Target

Kappa-type opioid receptor (CHEMBL3614)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Structure-activity study on the Phe side chain arrangement of endomorphins using conformationally constrained analogues.
Tömböly C, Kövér KE, Péter A, Tourwé D, Biyashev D, Benyhe S, Borsodi A, Al-Khrasani M, Rónai AZ, Tóth G.
J Med Chem (2004) 47 : 735 -743
PubMed 14736254 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 6.98 7.97

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL333357 ENDOMORPHIN 2 1 7.97
CHEMBL436039 1 7.92
CHEMBL163334 1 7.55
CHEMBL166312 1 7.47
CHEMBL316446 ENDOMORPHIN-1 1 7.28
CHEMBL423794 1 7.25
CHEMBL351976 1 7.18
CHEMBL423466 1 7.08
CHEMBL349738 1 7.03
CHEMBL355141 1 6.74
CHEMBL163871 1 6.50
CHEMBL355153 1 6.33
CHEMBL355367 1 6.00
CHEMBL166108 1 5.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL333357 ENDOMORPHIN 2 Ki = 10.6 nM 7.97
CHEMBL436039 Ki = 11.9 nM 7.92
CHEMBL163334 Ki = 28.1 nM 7.55
CHEMBL166312 Ki = 33.7 nM 7.47
CHEMBL316446 ENDOMORPHIN-1 Ki = 52.7 nM 7.28
CHEMBL423794 Ki = 55.7 nM 7.25
CHEMBL351976 Ki = 65.4 nM 7.18
CHEMBL423466 Ki = 83.0 nM 7.08
CHEMBL349738 Ki = 94.3 nM 7.03
CHEMBL355141 Ki = 181.0 nM 6.74
CHEMBL163871 Ki = 316.0 nM 6.50
CHEMBL355153 Ki = 467.0 nM 6.33
CHEMBL355367 Ki = 990.0 nM 6.00
CHEMBL166108 Ki = 4470.0 nM 5.35