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Assay Detail

CHEMBL759066

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to displace bound [20-3H]-PDBU from a recombinant single isozyme (PKCalpha) in the presence of phosphatidylserine
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Protein kinase C alpha type (CHEMBL2213)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

An optimized protein kinase C activating diacylglycerol combining high binding affinity (Ki) with reduced lipophilicity (log P).
Nacro K, Sigano DM, Yan S, Nicklaus MC, Pearce LL, Lewin NE, Garfield SH, Blumberg PM, Marquez VE.
J Med Chem (2001) 44 : 1892 -1904
PubMed 11384235 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 21 7.11 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL27768 1 9.70
CHEMBL170518 PROSTRATIN 1 8.32
CHEMBL294422 1 7.61
CHEMBL289230 1 7.54
CHEMBL38644 1 7.51
CHEMBL55267 1 7.48
CHEMBL40482 1 7.40
CHEMBL300160 1 7.35
CHEMBL59422 1 7.30
CHEMBL59376 1 7.17
CHEMBL293717 1 7.15
CHEMBL57015 1 7.14
CHEMBL292417 1 7.13
CHEMBL57517 DIOLEIN 1 7.06
CHEMBL56997 1 6.96
CHEMBL57400 1 6.81
CHEMBL40602 1 6.48
CHEMBL57211 1 5.98
CHEMBL292383 1 5.85
CHEMBL293709 1 5.75
CHEMBL57105 1 5.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL27768 Ki = 0.2 nM 9.70
CHEMBL170518 PROSTRATIN Ki = 4.8 nM 8.32
CHEMBL294422 Ki = 24.6 nM 7.61
CHEMBL289230 Ki = 28.6 nM 7.54
CHEMBL38644 Ki = 31.1 nM 7.51
CHEMBL55267 Ki = 33.0 nM 7.48
CHEMBL40482 Ki = 39.9 nM 7.40
CHEMBL300160 Ki = 44.5 nM 7.35
CHEMBL59422 Ki = 50.4 nM 7.30
CHEMBL59376 Ki = 68.3 nM 7.17
CHEMBL293717 Ki = 71.0 nM 7.15
CHEMBL57015 Ki = 73.0 nM 7.14
CHEMBL292417 Ki = 74.9 nM 7.13
CHEMBL57517 DIOLEIN Ki = 87.2 nM 7.06
CHEMBL56997 Ki = 110.0 nM 6.96
CHEMBL57400 Ki = 154.0 nM 6.81
CHEMBL40602 Ki = 330.0 nM 6.48
CHEMBL57211 Ki = 1050.0 nM 5.98
CHEMBL292383 Ki = 1409.0 nM 5.85
CHEMBL293709 Ki = 1800.0 nM 5.75
CHEMBL57105 Ki = 2400.0 nM 5.62