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Assay Detail

CHEMBL762145

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement [3H]LY-186,126 from phosphodiesterase 4 of myocardial vesicles
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Canis lupus familiaris
Tissue Myocardium
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Phosphodiesterase 4 (CHEMBL2093863)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Synthesis of a tritium-labeled indolidan analogue and its use as a radioligand for phosphodiesterase-inhibitor cardiotonic binding sites.
Robertson DW, Krushinski JH, Utterback BG, Kauffman RF.
J Med Chem (1989) 32 : 1476 -1480
PubMed 2738882 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 7.29 9.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL38017 1 9.27
CHEMBL38224 INDOLIDAN 2.0 1 8.27
CHEMBL34945 1 7.40
CHEMBL37981 1 4.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL38017 Ki = 0.54 nM 9.27
CHEMBL38224 INDOLIDAN Ki = 5.4 nM 8.27
CHEMBL34945 Ki = 40.0 nM 7.40
CHEMBL37981 Ki = 61400.0 nM 4.21