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Assay Detail

CHEMBL768512

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H-20]-phorbol-12-13-dibutyrate from bovine brain protein kinase C alpha
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Expert

Target

Protein kinase C alpha type (CHEMBL2213)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Conformationally constrained analogues of diacylglycerol. 9.1 the effect of side-chain orientation on the protein kinase C (PK-C) binding affinity of -lactones
Lee J, Lewin NE, Blumberg PM, Marquez VE
Bioorg Med Chem Lett (1994) 4 : 2405 -2410
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 6.04 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL78799 1 6.70
CHEMBL422215 1 6.70
CHEMBL13306 1 6.52
CHEMBL79376 1 5.89
CHEMBL81971 1 5.72
CHEMBL78679 1 4.69

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL78799 Ki = 200.0 nM 6.70
CHEMBL422215 Ki = 200.0 nM 6.70
CHEMBL13306 Ki = 300.0 nM 6.52
CHEMBL79376 Ki = 1300.0 nM 5.89
CHEMBL81971 Ki = 1900.0 nM 5.72
CHEMBL78679 Ki = 20500.0 nM 4.69