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Assay Detail

CHEMBL806327

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibition of [14C]- guanidinium influx in Chinese hamster ovary (CHO) cells expressing rat brain sodium channel type IIA (CNaIIA-1)
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

Sodium channel protein type 2 subunit alpha (CHEMBL3399)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Design, synthesis, and pharmacological evaluation of conformationally constrained analogues of N,N'-diaryl- and N-aryl-N-aralkylguanidines as potent inhibitors of neuronal Na+ channels.
Maillard MC, Perlman ME, Amitay O, Baxter D, Berlove D, Connaughton S, Fischer JB, Guo JQ, Hu LY, McBurney RN, Nagy PI, Subbarao K, Yost EA, Zhang L, Durant GJ.
J Med Chem (1998) 41 : 3048 -3061
PubMed 9685245 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.48 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL507974 TETRODOTOXIN 3.0 1 8.40
CHEMBL281724 LUBELUZOLE 2.0 1 6.58
CHEMBL544330 1 5.54
CHEMBL544569 1 5.48
CHEMBL542191 1 5.00
CHEMBL554829 1 4.83
CHEMBL553355 1 4.76
CHEMBL545737 1 4.42
CHEMBL543152 1 4.28
CHEMBL544329 1 -
CHEMBL537480 1 -
CHEMBL103505 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL507974 TETRODOTOXIN IC50 = 4.0 nM 8.40
CHEMBL281724 LUBELUZOLE IC50 = 260.0 nM 6.58
CHEMBL544330 IC50 = 2900.0 nM 5.54
CHEMBL544569 IC50 = 3280.0 nM 5.48
CHEMBL542191 IC50 = 9950.0 nM 5.00
CHEMBL554829 IC50 = 14680.0 nM 4.83
CHEMBL553355 IC50 = 17550.0 nM 4.76
CHEMBL545737 IC50 = 38020.0 nM 4.42
CHEMBL543152 IC50 = 53000.0 nM 4.28
CHEMBL544329 IC50 > 25000.0 nM -
CHEMBL537480 IC50 > 24000.0 nM -
CHEMBL103505 IC50 > 25000.0 nM -