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Assay Detail

CHEMBL808805

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of Subtilisin Carlsberg enzyme from Bacillus lentus
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lederbergia lenta
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Subtilisin Carlsberg (CHEMBL4299)
Type SINGLE PROTEIN
Organism Bacillus licheniformis

Publication

Probing the specificity of the S1 binding site of M222 mutants of subtilisin B. lentus with boronic acid inhibitors
Stabile MR, George Lai W, DeSantis G, Gold M, Bryan Jones J, Mitchinson C, Bott RR, Graycar TP, Liu C
Bioorg Med Chem Lett (1996) 6 : 2501 -2506
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 4.53 5.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL20945 1 5.10
CHEMBL20726 1 5.00
CHEMBL21447 1 4.72
CHEMBL20866 1 4.64
CHEMBL20421 1 4.43
CHEMBL79870 1 4.24
CHEMBL20852 1 4.12
CHEMBL21485 PHENYLBORONIC ACID 1 4.00
CHEMBL21848 1 -
CHEMBL20461 PHENETHYLBORONIC ACID 1 -
CHEMBL21427 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL20945 Ki = 8000.0 nM 5.10
CHEMBL20726 Ki = 10000.0 nM 5.00
CHEMBL21447 Ki = 19000.0 nM 4.72
CHEMBL20866 Ki = 23000.0 nM 4.64
CHEMBL20421 Ki = 37000.0 nM 4.43
CHEMBL79870 Ki = 58000.0 nM 4.24
CHEMBL20852 Ki = 75000.0 nM 4.12
CHEMBL21485 PHENYLBORONIC ACID Ki = 100000.0 nM 4.00
CHEMBL21848 Ki = 120000.0 nM -
CHEMBL20461 PHENETHYLBORONIC ACID Ki = 257000.0 nM -
CHEMBL21427 Ki = 180000.0 nM -