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Assay Detail

CHEMBL810541

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]glibenclamide binding to HEK293 cells co-expressing human Sulfonylurea receptor SUR1 and Inward rectifier K+ channel Kir6.2 at high affinity state with 2 mM MgATP
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Sulfonylurea receptor 1, Kir6.2 (CHEMBL2096972)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

6-Chloro-3-alkylamino-4H-thieno[3,2-e]-1,2,4-thiadiazine 1,1-dioxide derivatives potently and selectively activate ATP sensitive potassium channels of pancreatic beta-cells.
Nielsen FE, Bodvarsdottir TB, Worsaae A, MacKay P, Stidsen CE, Boonen HC, Pridal L, Arkhammar PO, Wahl P, Ynddal L, Junager F, Dragsted N, Tagmose TM, Mogensen JP, Koch A, Treppendahl SP, Hansen JB.
J Med Chem (2002) 45 : 4171 -4187
PubMed 12213059 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.66 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL134168 1 8.52
CHEMBL133663 1 6.92
CHEMBL135091 1 6.75
CHEMBL336586 1 6.64
CHEMBL135191 TIFENAZOXIDE 2.0 1 6.50
CHEMBL131971 1 6.47
CHEMBL3350270 1 6.34
CHEMBL2111887 1 6.19
CHEMBL133596 1 5.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL134168 IC50 = 3.0 nM 8.52
CHEMBL133663 IC50 = 120.0 nM 6.92
CHEMBL135091 IC50 = 180.0 nM 6.75
CHEMBL336586 IC50 = 230.0 nM 6.64
CHEMBL135191 TIFENAZOXIDE IC50 = 320.0 nM 6.50
CHEMBL131971 IC50 = 340.0 nM 6.47
CHEMBL3350270 IC50 = 460.0 nM 6.34
CHEMBL2111887 IC50 = 640.0 nM 6.19
CHEMBL133596 IC50 = 2270.0 nM 5.64