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Assay Detail

CHEMBL812190

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Concentration required to reverse inhibition of T-cell proliferation, by measuring [3H]thymidine uptake up to a concentration of 10 uM (Antagonistic activity, immunosuppression)
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type T-cell
Confidence 1 — Organism
Curated By Intermediate

Target

T-cell (CHEMBL614309)
Type CELL-LINE

Publication

Novel derivatives at the C21 position of the FK-506 macrocycle
Organ HM, Holmes MA, Pisano JM, Staruch MJ, Wyvratt MJ, Dumont FJ, Sinclair PJ
Bioorg Med Chem Lett (1993) 3 : 657 -662
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 10 6.75 7.93

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL347589 1 7.93
CHEMBL349585 1 7.47
CHEMBL423774 1 7.29
CHEMBL407238 1 7.19
CHEMBL348122 1 7.06
CHEMBL161068 1 6.67
CHEMBL350884 1 6.64
CHEMBL422171 1 6.00
CHEMBL161316 1 5.75
CHEMBL162354 1 5.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL347589 EC50 = 11.8 nM 7.93
CHEMBL349585 EC50 = 34.1 nM 7.47
CHEMBL423774 EC50 = 51.0 nM 7.29
CHEMBL407238 EC50 = 64.0 nM 7.19
CHEMBL348122 EC50 = 88.0 nM 7.06
CHEMBL161068 EC50 = 212.0 nM 6.67
CHEMBL350884 EC50 = 228.0 nM 6.64
CHEMBL422171 EC50 = 992.0 nM 6.00
CHEMBL161316 EC50 = 1780.0 nM 5.75
CHEMBL162354 EC50 = 3522.0 nM 5.45