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Assay Detail

CHEMBL812192

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonistic activity in a murine splenic T-cell proliferation assay was calculated by measurement of [3H]thymidine uptake relative to control
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type T-cell
Confidence 1 — Organism
Curated By Expert

Target

T-cell (CHEMBL614309)
Type CELL-LINE

Publication

Novel derivatives at the C21 position of the FK-506 macrocycle
Organ HM, Holmes MA, Pisano JM, Staruch MJ, Wyvratt MJ, Dumont FJ, Sinclair PJ
Bioorg Med Chem Lett (1993) 3 : 657 -662
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 7.04 9.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL269732 TACROLIMUS ANHYDROUS 4.0 1 9.74
CHEMBL8597 ASCOMYCIN 1 9.16
CHEMBL347139 1 9.03
CHEMBL422367 1 8.67
CHEMBL352172 1 8.58
CHEMBL351445 1 8.56
CHEMBL351140 1 8.30
CHEMBL348352 1 8.15
CHEMBL158960 1 7.77
CHEMBL346057 1 7.77
CHEMBL436659 1 7.69
CHEMBL422171 1 7.00
CHEMBL349585 1 5.93
CHEMBL347589 1 5.88
CHEMBL347657 1 5.78
CHEMBL158921 1 5.68
CHEMBL347383 1 5.47
CHEMBL161316 1 5.44
CHEMBL162354 1 5.37
CHEMBL350884 1 5.00
CHEMBL161068 1 4.94
CHEMBL348122 1 4.93
CHEMBL423774 1 -
CHEMBL407238 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL269732 TACROLIMUS ANHYDROUS IC50 = 0.18 nM 9.74
CHEMBL8597 ASCOMYCIN IC50 = 0.69 nM 9.16
CHEMBL347139 IC50 = 0.93 nM 9.03
CHEMBL422367 IC50 = 2.14 nM 8.67
CHEMBL352172 IC50 = 2.62 nM 8.58
CHEMBL351445 IC50 = 2.72 nM 8.56
CHEMBL351140 IC50 = 4.98 nM 8.30
CHEMBL348352 IC50 = 7.12 nM 8.15
CHEMBL158960 IC50 = 16.9 nM 7.77
CHEMBL346057 IC50 = 17.0 nM 7.77
CHEMBL436659 IC50 = 20.5 nM 7.69
CHEMBL422171 IC50 = 100.0 nM 7.00
CHEMBL349585 IC50 = 1182.0 nM 5.93
CHEMBL347589 IC50 = 1327.0 nM 5.88
CHEMBL347657 IC50 = 1677.0 nM 5.78
CHEMBL158921 IC50 = 2087.0 nM 5.68
CHEMBL347383 IC50 = 3400.0 nM 5.47
CHEMBL161316 IC50 = 3600.0 nM 5.44
CHEMBL162354 IC50 = 4248.0 nM 5.37
CHEMBL350884 IC50 = 10000.0 nM 5.00
CHEMBL161068 IC50 = 11400.0 nM 4.94
CHEMBL348122 IC50 = 11800.0 nM 4.93
CHEMBL423774 IC50 > 10000.0 nM -
CHEMBL407238 IC50 - nM -