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Assay Detail

CHEMBL813843

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory concentration of compound to inhibit Thymidylate synthase (TS) in L1210 cells at conc. of 200 uM
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L1210
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Thymidylate synthase (CHEMBL5328)
Type SINGLE PROTEIN
Organism Lactobacillus casei

Publication

Quinazoline antifolates inhibiting thymidylate synthase: 2-desamino derivatives with enhanced solubility and potency.
Jones TR, Thornton TJ, Flinn A, Jackman AL, Newell DR, Calvert AH.
J Med Chem (1989) 32 : 847 -852
PubMed 2704030 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.53 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL422395 1 7.70
CHEMBL22632 1 7.22
CHEMBL171480 1 6.80
CHEMBL170846 1 6.28
CHEMBL89641 1 4.64
CHEMBL23977 DIDEAZAFOLATE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL422395 IC50 = 20.0 nM 7.70
CHEMBL22632 IC50 = 60.0 nM 7.22
CHEMBL171480 IC50 = 160.0 nM 6.80
CHEMBL170846 IC50 = 520.0 nM 6.28
CHEMBL89641 IC50 = 22720.0 nM 4.64
CHEMBL23977 DIDEAZAFOLATE IC50 = 2.44 -