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Assay Detail

CHEMBL815661

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of thymidylate synthase purified from mouse L1210 leukemia cells that overproduce thymidylate synthase (TS)
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L1210
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Thymidylate synthase (CHEMBL3160)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Quinazoline antifolate thymidylate synthase inhibitors: replacement of glutamic acid in the C2-methyl series.
Marsham PR, Jackman AL, Barker AJ, Boyle FT, Pegg SJ, Wardleworth JM, Kimbell R, O'Connor BM, Calvert AH, Hughes LR.
J Med Chem (1995) 38 : 994 -1004
PubMed 7699716 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.60 8.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6271 1 8.36
CHEMBL414012 1 8.05
CHEMBL6636 1 7.62
CHEMBL266956 1 7.47
CHEMBL6676 1 7.40
CHEMBL6788 1 7.38
CHEMBL268629 1 7.38
CHEMBL268453 1 7.36
CHEMBL6663 1 7.24
CHEMBL267832 1 7.24
CHEMBL266509 1 7.17
CHEMBL6325 1 6.96
CHEMBL266508 1 6.82
CHEMBL415104 1 6.77
CHEMBL6850 1 6.57
CHEMBL268100 1 6.55
CHEMBL6775 1 6.50
CHEMBL6357 1 6.35
CHEMBL6560 1 6.28
CHEMBL266065 1 6.28
CHEMBL6829 1 6.14
CHEMBL6496 1 6.07
CHEMBL6590 1 5.86
CHEMBL264097 1 5.85
CHEMBL6317 1 5.84
CHEMBL415105 1 5.74
CHEMBL265410 1 5.65
CHEMBL6498 1 5.38
CHEMBL268854 1 5.11
CHEMBL6307 1 4.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6271 IC50 = 4.4 nM 8.36
CHEMBL414012 IC50 = 9.0 nM 8.05
CHEMBL6636 IC50 = 24.0 nM 7.62
CHEMBL266956 IC50 = 34.0 nM 7.47
CHEMBL6676 IC50 = 40.0 nM 7.40
CHEMBL6788 IC50 = 42.0 nM 7.38
CHEMBL268629 IC50 = 42.0 nM 7.38
CHEMBL268453 IC50 = 44.0 nM 7.36
CHEMBL6663 IC50 = 58.0 nM 7.24
CHEMBL267832 IC50 = 58.0 nM 7.24
CHEMBL266509 IC50 = 67.0 nM 7.17
CHEMBL6325 IC50 = 110.0 nM 6.96
CHEMBL266508 IC50 = 150.0 nM 6.82
CHEMBL415104 IC50 = 170.0 nM 6.77
CHEMBL6850 IC50 = 270.0 nM 6.57
CHEMBL268100 IC50 = 280.0 nM 6.55
CHEMBL6775 IC50 = 320.0 nM 6.50
CHEMBL6357 IC50 = 450.0 nM 6.35
CHEMBL6560 IC50 = 520.0 nM 6.28
CHEMBL266065 IC50 = 520.0 nM 6.28
CHEMBL6829 IC50 = 720.0 nM 6.14
CHEMBL6496 IC50 = 860.0 nM 6.07
CHEMBL6590 IC50 = 1380.0 nM 5.86
CHEMBL264097 IC50 = 1420.0 nM 5.85
CHEMBL6317 IC50 = 1460.0 nM 5.84
CHEMBL415105 IC50 = 1820.0 nM 5.74
CHEMBL265410 IC50 = 2260.0 nM 5.65
CHEMBL6498 IC50 = 4200.0 nM 5.38
CHEMBL268854 IC50 = 7700.0 nM 5.11
CHEMBL6307 IC50 = 22900.0 nM 4.64