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Assay Detail

CHEMBL815679

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of cellular thymidylate synthase activity of mouse fibroblast (L929 TK-) in intact cells.
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Thymidylate synthase (CHEMBL3160)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and biological activity of aromatic amino acid phosphoramidates of 5-fluoro-2'-deoxyuridine and 1-beta-arabinofuranosylcytosine: evidence of phosphoramidase activity.
Abraham TW, Kalman TI, McIntee EJ, Wagner CR.
J Med Chem (1996) 39 : 4569 -4575
PubMed 8917645 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.76 6.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1791078 1 6.17
CHEMBL96148 1 5.96
CHEMBL917 FLOXURIDINE 4.0 1 5.14
CHEMBL886 5-FLUORODEOXYURIDYLATE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1791078 IC50 = 670.0 nM 6.17
CHEMBL96148 IC50 = 1100.0 nM 5.96
CHEMBL917 FLOXURIDINE IC50 = 7300.0 nM 5.14
CHEMBL886 5-FLUORODEOXYURIDYLATE IC50 > 10000.0 nM -