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Assay Detail

CHEMBL817536

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Compound was evaluated for in vitro inhibitory activity against polymerization of tubulin into microtubules isolated from bovine brain at 1 mg/mL tubulin concentration
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Tubulin beta chain (CHEMBL3394)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Combretatroponeshybrids of combretastatin and colchicine. Synthesis and biochemical evaluation
Andres C, Bernardo J, Yan Q, Hastie S, Macdonald T
Bioorg Med Chem Lett (1993) 3 : 565 -570
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.32 5.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL79720 1 5.60
CHEMBL67 COMBRETASTATIN A4 -1.0 1 5.54
CHEMBL107 COLCHICINE 4.0 1 5.46
CHEMBL156658 1 5.07
CHEMBL154628 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL79720 IC50 = 2500.0 nM 5.60
CHEMBL67 COMBRETASTATIN A4 IC50 = 2900.0 nM 5.54
CHEMBL107 COLCHICINE IC50 = 3500.0 nM 5.46
CHEMBL156658 IC50 = 8600.0 nM 5.07
CHEMBL154628 IC50 = 12000.0 nM 4.92