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Assay Detail

CHEMBL818975

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibition of purified bovine brain tubulin polymerisation in the absence of microtubule-associated proteins.
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Tubulin (CHEMBL2094134)
Type PROTEIN FAMILY
Organism Bos taurus

Publication

Tubulin binding of conformationally restricted bis-aryl compounds
Huber SK, Werbovetz KA, Obaza-Nutaitis J, Lehnert EK, Macdonald TL
Bioorg Med Chem Lett (1991) 1 : 243 -246
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.24 5.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL10557 1 5.85
CHEMBL10121 1 5.82
CHEMBL10215 1 5.62
CHEMBL81233 1 5.62
CHEMBL107 COLCHICINE 4.0 1 5.60
CHEMBL61 PODOFILOX 4.0 1 5.60
CHEMBL81845 1 5.30
CHEMBL79591 1 5.28
CHEMBL10127 1 5.24
CHEMBL79720 1 5.12
CHEMBL82023 1 4.92
CHEMBL82024 1 4.17
CHEMBL81794 1 4.01
CHEMBL79497 1 -
CHEMBL81010 1 -
CHEMBL79985 1 -
CHEMBL309538 1 -
CHEMBL81280 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL10557 IC50 = 1400.0 nM 5.85
CHEMBL10121 IC50 = 1500.0 nM 5.82
CHEMBL10215 IC50 = 2400.0 nM 5.62
CHEMBL81233 IC50 = 2400.0 nM 5.62
CHEMBL107 COLCHICINE IC50 = 2500.0 nM 5.60
CHEMBL61 PODOFILOX IC50 = 2500.0 nM 5.60
CHEMBL81845 IC50 = 5000.0 nM 5.30
CHEMBL79591 IC50 = 5300.0 nM 5.28
CHEMBL10127 IC50 = 5800.0 nM 5.24
CHEMBL79720 IC50 = 7500.0 nM 5.12
CHEMBL82023 IC50 = 12000.0 nM 4.92
CHEMBL82024 IC50 = 67000.0 nM 4.17
CHEMBL81794 IC50 = 98000.0 nM 4.01
CHEMBL79497 IC50 > 200000.0 nM -
CHEMBL81010 IC50 > 125000.0 nM -
CHEMBL79985 IC50 = 104000.0 nM -
CHEMBL309538 IC50 > 200000.0 nM -
CHEMBL81280 IC50 = 150000.0 nM -