Assay Detail
Functional
CHEMBL819282
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Ability to inhibit WI-L2 murine leukemia tumor cell growth in culture after 48 hr of its exposure.
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | WI-L2 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Analogues of methotrexate and aminopterin with gamma-methylene and gamma-cyano substitution of the glutamate side chain: synthesis and in vitro biological activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.77 | 8.15 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL376180 | AMINOPTERIN | 2.0 | 1 | 8.15 |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.89 |
| CHEMBL65711 | — | — | 1 | 7.70 |
| CHEMBL65616 | — | — | 1 | 7.32 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL376180 | AMINOPTERIN | IC50 | = | 7.1 | nM | 8.15 |
| CHEMBL34259 | METHOTREXATE | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL65711 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL65616 | — | IC50 | = | 48.0 | nM | 7.32 |