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Assay Detail

CHEMBL820613

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Thymidylate synthase activity in L1210 cells
20
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L1210
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Thymidylate synthase (CHEMBL3160)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Quinazoline antifolate thymidylate synthase inhibitors: gamma-linked L-D, D-D, and D-L dipeptide analogues of 2-desamino-2-methyl-N10-propargyl-5,8-dideazafolic acid (ICI 198583).
Bavetsias V, Jackman AL, Kimbell R, Gibson W, Boyle FT, Bisset GM.
J Med Chem (1996) 39 : 73 -85
PubMed 8568829 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 8.23 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL170933 1 9.40
CHEMBL349432 1 9.25
CHEMBL354785 1 8.72
CHEMBL353066 1 8.72
CHEMBL168952 1 8.70
CHEMBL355543 1 8.70
CHEMBL170494 1 8.70
CHEMBL168970 2 8.38
CHEMBL170789 1 8.34
CHEMBL367732 1 8.34
CHEMBL263325 1 8.06
CHEMBL170975 1 7.92
CHEMBL168450 1 7.89
CHEMBL424541 1 7.77
CHEMBL352327 1 7.75
CHEMBL168553 1 7.57
CHEMBL352357 1 7.52
CHEMBL349488 1 7.47
CHEMBL169540 1 7.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL170933 IC50 = 0.4 nM 9.40
CHEMBL349432 IC50 = 0.56 nM 9.25
CHEMBL354785 IC50 = 1.9 nM 8.72
CHEMBL353066 IC50 = 1.9 nM 8.72
CHEMBL168952 IC50 = 2.0 nM 8.70
CHEMBL355543 IC50 = 2.0 nM 8.70
CHEMBL170494 IC50 = 2.0 nM 8.70
CHEMBL168970 IC50 = 4.2 nM 8.38
CHEMBL367732 IC50 = 4.6 nM 8.34
CHEMBL170789 IC50 = 4.6 nM 8.34
CHEMBL263325 IC50 = 8.8 nM 8.06
CHEMBL168970 IC50 = 10.0 nM 8.00
CHEMBL170975 IC50 = 12.0 nM 7.92
CHEMBL168450 IC50 = 13.0 nM 7.89
CHEMBL424541 IC50 = 17.0 nM 7.77
CHEMBL352327 IC50 = 18.0 nM 7.75
CHEMBL168553 IC50 = 27.0 nM 7.57
CHEMBL352357 IC50 = 30.0 nM 7.52
CHEMBL349488 IC50 = 34.0 nM 7.47
CHEMBL169540 IC50 = 37.0 nM 7.43