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Assay Detail

CHEMBL821264

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of thymidylate synthase, partially purified from L1210 mouse leukemia cells overexpressing TS
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type L1210
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Thymidylate synthase (CHEMBL3160)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Syntheses and thymidylate synthase inhibitory activity of the poly-gamma-glutamyl conjugates of N-[5-[N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino ]-2-thenoyl]-L-glutamic acid (ICI D1694) and other quinazoline antifolates.
Bisset GM, Pawelczak K, Jackman AL, Calvert AH, Hughes LR.
J Med Chem (1992) 35 : 859 -866
PubMed 1372358 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 8.50 9.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1202139 1 9.57
CHEMBL264807 1 9.40
CHEMBL1202137 1 9.40
CHEMBL171226 1 9.23
CHEMBL1202138 1 9.20
CHEMBL1202140 1 9.01
CHEMBL436448 1 9.00
CHEMBL355543 1 8.96
CHEMBL405513 1 8.43
CHEMBL268593 1 8.42
CHEMBL172160 1 8.33
CHEMBL355321 1 8.12
CHEMBL169981 1 7.98
CHEMBL353302 1 7.62
CHEMBL434209 1 7.50
CHEMBL96367 1 7.20
CHEMBL103059 1 7.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1202139 Ki = 0.27 nM 9.57
CHEMBL264807 Ki = 0.4 nM 9.40
CHEMBL1202137 Ki = 0.4 nM 9.40
CHEMBL171226 Ki = 0.59 nM 9.23
CHEMBL1202138 Ki = 0.63 nM 9.20
CHEMBL1202140 Ki = 0.97 nM 9.01
CHEMBL436448 Ki = 1.0 nM 9.00
CHEMBL355543 Ki = 1.11 nM 8.96
CHEMBL405513 Ki = 3.7 nM 8.43
CHEMBL268593 Ki = 3.79 nM 8.42
CHEMBL172160 Ki = 4.69 nM 8.33
CHEMBL355321 Ki = 7.52 nM 8.12
CHEMBL169981 Ki = 10.4 nM 7.98
CHEMBL353302 Ki = 24.0 nM 7.62
CHEMBL434209 Ki = 31.38 nM 7.50
CHEMBL96367 Ki = 63.7 nM 7.20
CHEMBL103059 Ki = 63.68 nM 7.20