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Assay Detail

CHEMBL828720

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [35S]TBPS from picrotoxin site of GABA-A receptor of rat brain membranes
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Neurosteroid analogues. 10. The effect of methyl group substitution at the C-6 and C-7 positions on the GABA modulatory and anesthetic actions of (3alpha,5alpha)- and (3alpha,5beta)-3-hydroxypregnan-20-one.
Zeng CM, Manion BD, Benz A, Evers AS, Zorumski CF, Mennerick S, Covey DF.
J Med Chem (2005) 48 : 3051 -3059
PubMed 15828844 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.15 8.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3138080 1 8.24
CHEMBL3138334 1 7.35
CHEMBL210952 ELTANOLONE 2.0 1 7.15
CHEMBL207538 BREXANOLONE 4.0 1 7.13
CHEMBL3138079 1 5.98
CHEMBL3138338 1 5.91
CHEMBL3138408 1 5.91
CHEMBL3138332 1 5.53
CHEMBL3138076 1 5.51
CHEMBL3138075 1 5.22
CHEMBL3138323 1 4.97
CHEMBL3138081 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3138080 IC50 = 5.7 nM 8.24
CHEMBL3138334 IC50 = 45.0 nM 7.35
CHEMBL210952 ELTANOLONE IC50 = 71.0 nM 7.15
CHEMBL207538 BREXANOLONE IC50 = 74.0 nM 7.13
CHEMBL3138079 IC50 = 1040.0 nM 5.98
CHEMBL3138338 IC50 = 1240.0 nM 5.91
CHEMBL3138408 IC50 = 1240.0 nM 5.91
CHEMBL3138332 IC50 = 2940.0 nM 5.53
CHEMBL3138076 IC50 = 3080.0 nM 5.51
CHEMBL3138075 IC50 = 5990.0 nM 5.22
CHEMBL3138323 IC50 = 10800.0 nM 4.97
CHEMBL3138081 IC50 = 12000.0 nM 4.92