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Assay Detail

CHEMBL831528

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]estradiol binding to human estrogen receptor alpha expressed in HeLa cells
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Expert

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective estrogen receptor modulators with conformationally restricted side chains. Synthesis and structure-activity relationship of ERalpha-selective tetrahydroisoquinoline ligands.
Renaud J, Bischoff SF, Buhl T, Floersheim P, Fournier B, Geiser M, Halleux C, Kallen J, Keller H, Ramage P.
J Med Chem (2005) 48 : 364 -379
PubMed 15658851 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.12 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL328190 LASOFOXIFENE 4.0 1 8.40
CHEMBL101382 1 7.72
CHEMBL81 RALOXIFENE 4.0 1 7.66
CHEMBL135 ESTRADIOL 4.0 1 7.55
CHEMBL195216 1 7.42
CHEMBL195212 1 7.20
CHEMBL195465 1 7.16
CHEMBL364492 1 7.08
CHEMBL192187 1 7.06
CHEMBL426461 1 6.97
CHEMBL372029 1 6.97
CHEMBL195118 1 6.92
CHEMBL191711 1 6.91
CHEMBL195466 1 6.91
CHEMBL372011 1 6.79
CHEMBL192055 1 6.79
CHEMBL194334 1 6.77
CHEMBL191646 1 6.75
CHEMBL427533 1 6.73
CHEMBL103650 1 6.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL328190 LASOFOXIFENE IC50 = 4.0 nM 8.40
CHEMBL101382 IC50 = 19.0 nM 7.72
CHEMBL81 RALOXIFENE IC50 = 22.0 nM 7.66
CHEMBL135 ESTRADIOL IC50 = 28.0 nM 7.55
CHEMBL195216 IC50 = 38.0 nM 7.42
CHEMBL195212 IC50 = 63.0 nM 7.20
CHEMBL195465 IC50 = 69.0 nM 7.16
CHEMBL364492 IC50 = 83.0 nM 7.08
CHEMBL192187 IC50 = 87.0 nM 7.06
CHEMBL426461 IC50 = 106.0 nM 6.97
CHEMBL372029 IC50 = 108.0 nM 6.97
CHEMBL195118 IC50 = 119.0 nM 6.92
CHEMBL191711 IC50 = 122.0 nM 6.91
CHEMBL195466 IC50 = 123.0 nM 6.91
CHEMBL372011 IC50 = 163.0 nM 6.79
CHEMBL192055 IC50 = 162.0 nM 6.79
CHEMBL194334 IC50 = 169.0 nM 6.77
CHEMBL191646 IC50 = 177.0 nM 6.75
CHEMBL427533 IC50 = 185.0 nM 6.73
CHEMBL103650 IC50 = 285.0 nM 6.54