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Assay Detail

CHEMBL832383

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 17-beta-estradiol mediated luciferase transcription in HeLa cells expressing human estrogen receptor beta; ERE assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Expert

Target

Estrogen receptor beta (CHEMBL242)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective estrogen receptor modulators with conformationally restricted side chains. Synthesis and structure-activity relationship of ERalpha-selective tetrahydroisoquinoline ligands.
Renaud J, Bischoff SF, Buhl T, Floersheim P, Fournier B, Geiser M, Halleux C, Kallen J, Keller H, Ramage P.
J Med Chem (2005) 48 : 364 -379
PubMed 15658851 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.57 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL328190 LASOFOXIFENE 4.0 1 7.62
CHEMBL195212 1 7.01
CHEMBL195465 1 6.97
CHEMBL192187 1 6.79
CHEMBL427533 1 6.75
CHEMBL195466 1 6.57
CHEMBL191646 1 6.56
CHEMBL364492 1 6.54
CHEMBL101382 1 6.54
CHEMBL194334 1 6.54
CHEMBL81 RALOXIFENE 4.0 1 6.47
CHEMBL195216 1 6.40
CHEMBL426461 1 6.33
CHEMBL195118 1 6.31
CHEMBL372029 1 6.30
CHEMBL192055 1 5.35
CHEMBL372011 1 -
CHEMBL83 TAMOXIFEN 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL328190 LASOFOXIFENE IC50 = 24.0 nM 7.62
CHEMBL195212 IC50 = 98.0 nM 7.01
CHEMBL195465 IC50 = 106.0 nM 6.97
CHEMBL192187 IC50 = 162.0 nM 6.79
CHEMBL427533 IC50 = 180.0 nM 6.75
CHEMBL195466 IC50 = 268.0 nM 6.57
CHEMBL191646 IC50 = 276.0 nM 6.56
CHEMBL364492 IC50 = 286.0 nM 6.54
CHEMBL101382 IC50 = 289.0 nM 6.54
CHEMBL194334 IC50 = 287.0 nM 6.54
CHEMBL81 RALOXIFENE IC50 = 341.0 nM 6.47
CHEMBL195216 IC50 = 399.0 nM 6.40
CHEMBL426461 IC50 = 464.0 nM 6.33
CHEMBL195118 IC50 = 491.0 nM 6.31
CHEMBL372029 IC50 = 496.0 nM 6.30
CHEMBL192055 IC50 = 4454.0 nM 5.35
CHEMBL372011 IC50 > 1000.0 nM -
CHEMBL83 TAMOXIFEN IC50 > 1000.0 nM -