Assay Detail
Functional
CHEMBL837231
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Growth inhibitory activity against MTX resistant CCRF-CEM human lymphoblastic leukemia cells with decreased polyglutamylation
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | CCRF-CEM |
| Confidence | 1 — Organism |
| Curated By | Intermediate |
Publication
Synthesis of classical, four-carbon bridged 5-substituted furo[2,3-d]pyrimidine and 6-substituted pyrrolo[2,3-d]pyrimidine analogues as antifolates.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 5 | 6.30 | 7.84 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.84 |
| CHEMBL223279 | — | — | 1 | 7.08 |
| CHEMBL192632 | — | — | 1 | 6.33 |
| CHEMBL193275 | — | — | 1 | 5.50 |
| CHEMBL365307 | — | — | 1 | 4.75 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | EC50 | = | 14.5 | nM | 7.84 |
| CHEMBL223279 | — | EC50 | = | 84.0 | nM | 7.08 |
| CHEMBL192632 | — | EC50 | = | 470.0 | nM | 6.33 |
| CHEMBL193275 | — | EC50 | = | 3150.0 | nM | 5.50 |
| CHEMBL365307 | — | EC50 | = | 18000.0 | nM | 4.75 |