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Assay Detail

CHEMBL839735

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 2-[125I]iodomelatonin binding to human melatonin receptor MT2 expressed in NIH3T3 rat fibroblast cells
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type NIH3T3
Confidence 9 — Direct single protein target
Curated By Expert

Target

Melatonin receptor type 1B (CHEMBL1946)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Analysis of structure-activity relationships for MT2 selective antagonists by melatonin MT1 and MT2 receptor models.
Rivara S, Lorenzi S, Mor M, Plazzi PV, Spadoni G, Bedini A, Tarzia G.
J Med Chem (2005) 48 : 4049 -4060
PubMed 15943478 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 8.05 10.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL285718 1 10.80
CHEMBL33549 1 9.49
CHEMBL274438 1 9.30
CHEMBL189449 1 9.18
CHEMBL34327 1 8.19
CHEMBL33185 1 8.06
CHEMBL187525 1 8.01
CHEMBL366004 1 6.54
CHEMBL372243 1 5.74
CHEMBL195074 1 5.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL285718 Ki = 0.01585 nM 10.80
CHEMBL33549 Ki = 0.3236 nM 9.49
CHEMBL274438 Ki = 0.5012 nM 9.30
CHEMBL189449 Ki = 0.6607 nM 9.18
CHEMBL34327 Ki = 6.457 nM 8.19
CHEMBL33185 Ki = 8.71 nM 8.06
CHEMBL187525 Ki = 9.772 nM 8.01
CHEMBL366004 Ki = 288.4 nM 6.54
CHEMBL372243 Ki = 1819.7 nM 5.74
CHEMBL195074 Ki = 6025.6 nM 5.22