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Assay Detail

CHEMBL841329

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity towards nAChR (alpha4-beta2) by the displacement of [3H](-)-cytisine in whole rat brain membranes.
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Subcellular Brain membranes
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Synthesis and structure-activity relationships of pyridine-modified analogs of 3-[2-((S)-pyrrolidinyl)methoxy]pyridine, A-84543, a potent nicotinic acetylcholine receptor agonist.
Lin NH, Gunn DE, Li Y, He Y, Bai H, Ryther KB, Kuntzweiler T, Donnelly-Roberts DL, Anderson DJ, Campbell JE, Sullivan JP, Arneric SP, Holladay MW.
Bioorg Med Chem Lett (1998) 8 : 249 -254
PubMed 9871663 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 31 8.34 9.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL86079 1 9.92
CHEMBL84149 1 9.82
CHEMBL312964 1 9.80
CHEMBL83009 1 9.68
CHEMBL82494 1 9.57
CHEMBL85998 1 9.49
CHEMBL86286 1 9.49
CHEMBL83473 1 9.32
CHEMBL82322 1 9.22
CHEMBL312810 1 9.20
CHEMBL84887 1 9.19
CHEMBL83133 1 9.16
CHEMBL85555 1 8.92
CHEMBL86162 1 8.90
CHEMBL85755 1 8.84
CHEMBL86004 1 8.74
CHEMBL84827 1 8.72
CHEMBL86212 1 8.59
CHEMBL84537 1 8.43
CHEMBL315212 1 8.38
CHEMBL85778 1 8.33
CHEMBL82954 1 7.70
CHEMBL84692 1 7.64
CHEMBL85956 1 7.55
CHEMBL84936 1 7.46
CHEMBL412372 1 7.01
CHEMBL312962 1 6.35
CHEMBL314922 1 6.25
CHEMBL86055 1 6.22
CHEMBL310893 1 5.55

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL86079 Ki = 0.12 nM 9.92
CHEMBL84149 Ki = 0.15 nM 9.82
CHEMBL312964 Ki = 0.16 nM 9.80
CHEMBL83009 Ki = 0.21 nM 9.68
CHEMBL82494 Ki = 0.27 nM 9.57
CHEMBL85998 Ki = 0.32 nM 9.49
CHEMBL86286 Ki = 0.32 nM 9.49
CHEMBL83473 Ki = 0.48 nM 9.32
CHEMBL82322 Ki = 0.6 nM 9.22
CHEMBL312810 Ki = 0.63 nM 9.20
CHEMBL84887 Ki = 0.64 nM 9.19
CHEMBL83133 Ki = 0.69 nM 9.16
CHEMBL85555 Ki = 1.19 nM 8.92
CHEMBL86162 Ki = 1.26 nM 8.90
CHEMBL85755 Ki = 1.45 nM 8.84
CHEMBL86004 Ki = 1.83 nM 8.74
CHEMBL84827 Ki = 1.91 nM 8.72
CHEMBL86212 Ki = 2.56 nM 8.59
CHEMBL84537 Ki = 3.7 nM 8.43
CHEMBL315212 Ki = 4.12 nM 8.38
CHEMBL85778 Ki = 4.65 nM 8.33
CHEMBL82954 Ki = 19.9 nM 7.70
CHEMBL84692 Ki = 22.9 nM 7.64
CHEMBL85956 Ki = 28.0 nM 7.55
CHEMBL84936 Ki = 34.8 nM 7.46
CHEMBL412372 Ki = 97.35 nM 7.01
CHEMBL312962 Ki = 451.0 nM 6.35
CHEMBL314922 Ki = 559.0 nM 6.25
CHEMBL86055 Ki = 606.0 nM 6.22
CHEMBL310893 Ki = 2833.0 nM 5.55
CHEMBL314597 Ki = 9000.0 nM 5.05