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Assay Detail

CHEMBL846887

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibitory activity on N-ethylcarboxamidoadenosine (NECA)-induced glucose production in primary cultured rat hepatocytes
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type Hepatocyte
Confidence 9 — Direct single protein target
Curated By Expert

Target

Adenosine receptor A2b (CHEMBL2592)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

2-Alkynyl-8-aryl-9-methyladenines as novel adenosine receptor antagonists: their synthesis and structure-activity relationships toward hepatic glucose production induced via agonism of the A(2B) receptor.
Harada H, Asano O, Hoshino Y, Yoshikawa S, Matsukura M, Kabasawa Y, Niijima J, Kotake Y, Watanabe N, Kawata T, Inoue T, Horizoe T, Yasuda N, Minami H, Nagata K, Murakami M, Nagaoka J, Kobayashi S, Tanaka I, Abe S.
J Med Chem (2001) 44 : 170 -179
PubMed 11170626 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.02 6.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL50051 1 6.66
CHEMBL48153 1 6.60
CHEMBL49925 1 6.39
CHEMBL297685 1 6.38
CHEMBL296389 1 6.36
CHEMBL301512 1 6.36
CHEMBL51107 1 6.31
CHEMBL300624 1 6.29
CHEMBL51192 1 6.27
CHEMBL299548 1 6.14
CHEMBL48683 1 6.12
CHEMBL51394 1 6.12
CHEMBL50950 1 6.01
CHEMBL62350 8-PHENYL THEOPHYLLINE 1 5.96
CHEMBL48681 1 5.92
CHEMBL51829 1 5.89
CHEMBL297949 1 5.80
CHEMBL52432 1 5.36
CHEMBL48682 1 5.28
CHEMBL51353 1 5.20
CHEMBL440115 FK453 1.0 1 5.09
CHEMBL52276 1 -
CHEMBL52735 1 -
CHEMBL50839 1 -
CHEMBL27508 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL50051 IC50 = 220.0 nM 6.66
CHEMBL48153 IC50 = 250.0 nM 6.60
CHEMBL49925 IC50 = 410.0 nM 6.39
CHEMBL297685 IC50 = 420.0 nM 6.38
CHEMBL296389 IC50 = 440.0 nM 6.36
CHEMBL301512 IC50 = 440.0 nM 6.36
CHEMBL51107 IC50 = 490.0 nM 6.31
CHEMBL300624 IC50 = 510.0 nM 6.29
CHEMBL51192 IC50 = 540.0 nM 6.27
CHEMBL299548 IC50 = 720.0 nM 6.14
CHEMBL48683 IC50 = 750.0 nM 6.12
CHEMBL51394 IC50 = 760.0 nM 6.12
CHEMBL50950 IC50 = 970.0 nM 6.01
CHEMBL62350 8-PHENYL THEOPHYLLINE IC50 = 1100.0 nM 5.96
CHEMBL48681 IC50 = 1200.0 nM 5.92
CHEMBL51829 IC50 = 1300.0 nM 5.89
CHEMBL297949 IC50 = 1600.0 nM 5.80
CHEMBL52432 IC50 = 4400.0 nM 5.36
CHEMBL48682 IC50 = 5300.0 nM 5.28
CHEMBL51353 IC50 = 6300.0 nM 5.20
CHEMBL440115 FK453 IC50 = 8100.0 nM 5.09
CHEMBL52276 IC50 > 10000.0 nM -
CHEMBL52735 IC50 > 10000.0 nM -
CHEMBL50839 IC50 > 10000.0 nM -
CHEMBL27508 IC50 > 10000.0 nM -