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Assay Detail

CHEMBL853360

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Binding
Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells
27
Total Activities
23
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SK-N-MC
Confidence 9 — Direct single protein target
Curated By Expert

Target

Histamine H4 receptor (CHEMBL3759)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Characterization of the histamine H4 receptor binding site. Part 1. Synthesis and pharmacological evaluation of dibenzodiazepine derivatives.
Smits RA, Lim HD, Stegink B, Bakker RA, de Esch IJ, Leurs R.
J Med Chem (2006) 49 : 4512 -4516
PubMed 16854056 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 23 6.65 7.80
Kd 2 7.95 7.97
Bmax 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL213912 3 7.97
CHEMBL42 CLOZAPINE 4.0 3 7.92
CHEMBL129198 JNJ-7777120 1 7.80
CHEMBL209758 1 7.56
CHEMBL209628 1 7.51
CHEMBL387054 1 7.41
CHEMBL7828 ISOLOXAPINE 1 7.37
CHEMBL211170 1 7.34
CHEMBL211242 1 7.10
CHEMBL339414 1 6.88
CHEMBL379102 1 6.72
CHEMBL1795042 1 6.54
CHEMBL378788 1 6.51
CHEMBL1794919 1 6.49
CHEMBL377985 1 5.97
CHEMBL266723 1 5.85
CHEMBL67284 ISOCLOTHIAPINE 1 5.69
CHEMBL831 LOXAPINE 4.0 1 5.34
CHEMBL386333 1 5.33
CHEMBL340801 PERLAPINE 2.0 1 5.32
CHEMBL377765 1 -
CHEMBL209680 1 -
CHEMBL90882 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL213912 Kd = 10.7 nM 7.97
CHEMBL42 CLOZAPINE Kd = 11.9 nM 7.92
CHEMBL129198 JNJ-7777120 Ki = 15.85 nM 7.80
CHEMBL209758 Ki = 27.54 nM 7.56
CHEMBL213912 Ki = 28.18 nM 7.55
CHEMBL209628 Ki = 30.9 nM 7.51
CHEMBL387054 Ki = 38.9 nM 7.41
CHEMBL7828 ISOLOXAPINE Ki = 42.66 nM 7.37
CHEMBL211170 Ki = 45.71 nM 7.34
CHEMBL211242 Ki = 79.43 nM 7.10
CHEMBL339414 Ki = 131.83 nM 6.88
CHEMBL42 CLOZAPINE Ki = 177.83 nM 6.75
CHEMBL379102 Ki = 190.55 nM 6.72
CHEMBL1795042 Ki = 288.4 nM 6.54
CHEMBL378788 Ki = 309.03 nM 6.51
CHEMBL1794919 Ki = 323.59 nM 6.49
CHEMBL377985 Ki = 1071.52 nM 5.97
CHEMBL266723 Ki = 1412.54 nM 5.85
CHEMBL67284 ISOCLOTHIAPINE Ki = 2041.74 nM 5.69
CHEMBL831 LOXAPINE Ki = 4570.88 nM 5.34
CHEMBL386333 Ki = 4677.35 nM 5.33
CHEMBL340801 PERLAPINE Ki = 4786.3 nM 5.32
CHEMBL90882 Ki < 10000.0 nM -
CHEMBL377765 Ki < 10000.0 nM -
CHEMBL209680 Ki < 10000.0 nM -
CHEMBL42 CLOZAPINE Bmax = 1011.0 fmol/mg -
CHEMBL213912 Bmax = 1034.0 fmol/mg -