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Assay Detail

CHEMBL854499

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Binding affinity to FXR assessed as ligand-dependent SRC1 recruitment by FRET based co-activator assay
13
Total Activities
12
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Expert

Target

Bile acid receptor (CHEMBL2047)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Back door modulation of the farnesoid X receptor: design, synthesis, and biological evaluation of a series of side chain modified chenodeoxycholic acid derivatives.
Pellicciari R, Gioiello A, Costantino G, Sadeghpour BM, Rizzo G, Meyer U, Parks DJ, Entrena-Guadix A, Fiorucci S.
J Med Chem (2006) 49 : 4208 -4215
PubMed 16821780 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 5.84 7.01
Efficacy 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL566315 OBETICHOLIC ACID 4.0 1 7.01
CHEMBL3138428 1 6.39
CHEMBL3138092 1 6.23
CHEMBL3138089 1 6.22
CHEMBL3138090 1 6.10
CHEMBL3138339 1 5.94
CHEMBL3138093 1 5.83
CHEMBL3138341 1 5.60
CHEMBL3138403 1 5.57
CHEMBL3138091 1 5.15
CHEMBL240597 CHENODIOL 4.0 2 5.06
CHEMBL3138044 1 5.01

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL566315 OBETICHOLIC ACID EC50 = 98.0 nM 7.01
CHEMBL3138428 EC50 = 410.0 nM 6.39
CHEMBL3138092 EC50 = 590.0 nM 6.23
CHEMBL3138089 EC50 = 600.0 nM 6.22
CHEMBL3138090 EC50 = 790.0 nM 6.10
CHEMBL3138339 EC50 = 1140.0 nM 5.94
CHEMBL3138093 EC50 = 1480.0 nM 5.83
CHEMBL3138341 EC50 = 2500.0 nM 5.60
CHEMBL3138403 EC50 = 2720.0 nM 5.57
CHEMBL3138091 EC50 = 7110.0 nM 5.15
CHEMBL240597 CHENODIOL EC50 = 8660.0 nM 5.06
CHEMBL3138044 EC50 = 9680.0 nM 5.01
CHEMBL240597 CHENODIOL Efficacy = 100.0 % -