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Assay Detail

CHEMBL856311

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human NHE1 expressed in Ap1 cell line
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Ap1
Confidence 9 — Direct single protein target
Curated By Expert

Target

Sodium/hydrogen exchanger 1 (CHEMBL2781)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological activity of 5-aryl-4-(4-(5-methyl-1H-imidazol-4-yl)piperidin-1-yl)pyrimidine analogs as potent, highly selective, and orally bioavailable NHE-1 inhibitors.
Atwal KS, O'Neil SV, Ahmad S, Doweyko L, Kirby M, Dorso CR, Chandrasena G, Chen BC, Zhao R, Zahler R.
Bioorg Med Chem Lett (2006) 16 : 4796 -4799
PubMed 16870436 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.78 8.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL384143 1 8.34
CHEMBL211924 1 8.27
CHEMBL217698 1 8.19
CHEMBL213568 1 8.13
CHEMBL385944 1 7.82
CHEMBL376979 1 7.68
CHEMBL212033 1 7.57
CHEMBL380382 1 7.39
CHEMBL384466 1 7.21
CHEMBL215399 1 7.21
CHEMBL214646 1 7.06
CHEMBL215510 1 6.60
CHEMBL425073 1 6.46
CHEMBL450633 1 6.46
CHEMBL64360 ENIPORIDE 2.0 1 6.40
CHEMBL215509 1 6.28
CHEMBL215928 1 6.24
CHEMBL211922 1 6.09
CHEMBL212856 1 6.03
CHEMBL214922 1 6.00
CHEMBL379871 1 5.72
CHEMBL436559 CARIPORIDE 2.0 1 5.46
CHEMBL384410 1 5.07
CHEMBL378339 1 4.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL384143 IC50 = 4.6 nM 8.34
CHEMBL211924 IC50 = 5.4 nM 8.27
CHEMBL217698 IC50 = 6.5 nM 8.19
CHEMBL213568 IC50 = 7.4 nM 8.13
CHEMBL385944 IC50 = 15.0 nM 7.82
CHEMBL376979 IC50 = 21.0 nM 7.68
CHEMBL212033 IC50 = 27.0 nM 7.57
CHEMBL380382 IC50 = 41.0 nM 7.39
CHEMBL384466 IC50 = 61.0 nM 7.21
CHEMBL215399 IC50 = 61.0 nM 7.21
CHEMBL214646 IC50 = 87.0 nM 7.06
CHEMBL215510 IC50 = 250.0 nM 6.60
CHEMBL425073 IC50 = 350.0 nM 6.46
CHEMBL450633 IC50 = 350.0 nM 6.46
CHEMBL64360 ENIPORIDE IC50 = 400.0 nM 6.40
CHEMBL215509 IC50 = 520.0 nM 6.28
CHEMBL215928 IC50 = 580.0 nM 6.24
CHEMBL211922 IC50 = 820.0 nM 6.09
CHEMBL212856 IC50 = 940.0 nM 6.03
CHEMBL214922 IC50 = 1000.0 nM 6.00
CHEMBL379871 IC50 = 1900.0 nM 5.72
CHEMBL436559 CARIPORIDE IC50 = 3500.0 nM 5.46
CHEMBL384410 IC50 = 8600.0 nM 5.07
CHEMBL378339 IC50 = 11000.0 nM 4.96