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Assay Detail

CHEMBL857513

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Apparent binding affinity against Dihydrofolate reductase in Escherichia coli
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL1809)
Type SINGLE PROTEIN
Organism Escherichia coli

Publication

2,4-Diamino-5-benzylpyrimidines and analogues as antibacterial agents. 12. 1,2-Dihydroquinolylmethyl analogues with high activity and specificity for bacterial dihydrofolate reductase.
Johnson JV, Rauchman BS, Baccanari DP, Roth B.
J Med Chem (1989) 32 : 1942 -1949
PubMed 2666668 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 9.21 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL418492 1 9.52
CHEMBL22 TRIMETHOPRIM 4.0 1 8.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL418492 Ki = 0.3 nM 9.52
CHEMBL22 TRIMETHOPRIM Ki = 1.3 nM 8.89