Assay Detail
Functional
CHEMBL858804
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of growth in MTX-resistant CCRF-CEM cell line, R30dm cells
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | CCRF-CEM |
| Confidence | 1 — Organism |
| Curated By | Expert |
Publication
Dual inhibitors of thymidylate synthase and dihydrofolate reductase as antitumor agents: design, synthesis, and biological evaluation of classical and nonclassical pyrrolo[2,3-d]pyrimidine antifolates(1).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 6.47 | 7.77 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.77 |
| CHEMBL225180 | — | — | 1 | 6.09 |
| CHEMBL371860 | — | — | 1 | 5.54 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | EC50 | = | 17.0 | nM | 7.77 |
| CHEMBL225180 | — | EC50 | = | 810.0 | nM | 6.09 |
| CHEMBL371860 | — | EC50 | = | 2900.0 | nM | 5.54 |