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Assay Detail

CHEMBL861291

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of [35S]GTP-gamma-S binding to rat mu opioid receptor expressed in CHO cells
30
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

Mu-type opioid receptor (CHEMBL270)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Design and synthesis of novel hydrazide-linked bifunctional peptides as delta/mu opioid receptor agonists and CCK-1/CCK-2 receptor antagonists.
Lee YS, Agnes RS, Badghisi H, Davis P, Ma SW, Lai J, Porreca F, Hruby VJ.
J Med Chem (2006) 49 : 1773 -1780
PubMed 16509592 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 20 7.72 9.19
Emax 10 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL374325 3 9.19
CHEMBL408889 3 8.29
CHEMBL405192 3 8.02
CHEMBL375412 3 8.02
CHEMBL408832 3 8.00
CHEMBL412331 3 7.84
CHEMBL38874 DAMGO 3 7.44
CHEMBL442483 3 7.12
CHEMBL413644 3 6.69
CHEMBL438739 3 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL374325 EC50 = 0.6457 nM 9.19
CHEMBL374325 EC50 = 0.64 nM 9.19
CHEMBL408889 EC50 = 5.129 nM 8.29
CHEMBL408889 EC50 = 5.2 nM 8.28
CHEMBL405192 EC50 = 9.55 nM 8.02
CHEMBL405192 EC50 = 9.5 nM 8.02
CHEMBL375412 EC50 = 9.6 nM 8.02
CHEMBL375412 EC50 = 9.772 nM 8.01
CHEMBL408832 EC50 = 10.0 nM 8.00
CHEMBL408832 EC50 = 10.47 nM 7.98
CHEMBL412331 EC50 = 14.45 nM 7.84
CHEMBL412331 EC50 = 15.0 nM 7.82
CHEMBL38874 DAMGO EC50 = 36.31 nM 7.44
CHEMBL38874 DAMGO EC50 = 37.0 nM 7.43
CHEMBL442483 EC50 = 76.0 nM 7.12
CHEMBL442483 EC50 = 75.86 nM 7.12
CHEMBL413644 EC50 = 204.17 nM 6.69
CHEMBL413644 EC50 = 210.0 nM 6.68
CHEMBL438739 EC50 = 239.88 nM 6.62
CHEMBL438739 EC50 = 240.0 nM 6.62
CHEMBL442483 Emax = 90.0 % -
CHEMBL374325 Emax = 130.0 % -
CHEMBL405192 Emax = 83.0 % -
CHEMBL408889 Emax = 84.0 % -
CHEMBL412331 Emax = 33.0 % -
CHEMBL375412 Emax = 82.0 % -
CHEMBL438739 Emax = 60.0 % -
CHEMBL38874 DAMGO Emax = 150.0 % -
CHEMBL413644 Emax = 170.0 % -
CHEMBL408832 Emax = 103.0 % -