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Assay Detail

CHEMBL862544

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]ZM-241385 from human adenosine A2A receptor expressed in HEK293 cells
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological studies of a new series of 5-heteroarylcarbamoylaminopyrazolo[4,3-e]1,2,4-triazolo[1,5-c]pyrimidines as human A3 adenosine receptor antagonists. Influence of the heteroaryl substituent on binding affinity and molecular modeling investigations.
Pastorin G, Da Ros T, Bolcato C, Montopoli C, Moro S, Cacciari B, Baraldi PG, Varani K, Borea PA, Spalluto G.
J Med Chem (2006) 49 : 1720 -1729
PubMed 16509587 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 6.78 7.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL203660 1 7.38
CHEMBL203054 1 7.22
CHEMBL383689 1 7.19
CHEMBL332091 1 7.19
CHEMBL203060 1 7.12
CHEMBL203998 1 6.96
CHEMBL118923 1 6.96
CHEMBL203797 1 6.90
CHEMBL383714 1 6.85
CHEMBL382357 1 6.82
CHEMBL534489 1 6.81
CHEMBL534936 1 6.75
CHEMBL204128 1 6.55
CHEMBL382665 1 6.46
CHEMBL381123 1 6.40
CHEMBL204753 1 6.39
CHEMBL262987 1 6.09
CHEMBL203506 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL203660 Ki = 42.0 nM 7.38
CHEMBL203054 Ki = 60.0 nM 7.22
CHEMBL383689 Ki = 64.0 nM 7.19
CHEMBL332091 Ki = 65.0 nM 7.19
CHEMBL203060 Ki = 75.0 nM 7.12
CHEMBL203998 Ki = 110.0 nM 6.96
CHEMBL118923 Ki = 110.0 nM 6.96
CHEMBL203797 Ki = 125.0 nM 6.90
CHEMBL383714 Ki = 140.0 nM 6.85
CHEMBL382357 Ki = 153.0 nM 6.82
CHEMBL534489 Ki = 155.0 nM 6.81
CHEMBL534936 Ki = 180.0 nM 6.75
CHEMBL204128 Ki = 282.0 nM 6.55
CHEMBL382665 Ki = 350.0 nM 6.46
CHEMBL381123 Ki = 400.0 nM 6.40
CHEMBL204753 Ki = 410.0 nM 6.39
CHEMBL262987 Ki = 810.0 nM 6.09
CHEMBL203506 Ki = 920.0 nM 6.04