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Assay Detail

CHEMBL872297

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Varicella-Zoster virus DNA polymerase (95 uL) activity in a solution containing 6.4 mM HEPES (pH 7.5), incubation for 12 minutes at 26 degrees C
12
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human alphaherpesvirus 3
Confidence 8 — Homologous single protein target
Curated By Expert

Target

DNA polymerase catalytic subunit (CHEMBL4859)
Type SINGLE PROTEIN
Organism Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)

Publication

4-Oxo-4,7-dihydrothieno[2,3-b]pyridines as non-nucleoside inhibitors of human cytomegalovirus and related herpesvirus polymerases.
Schnute ME, Cudahy MM, Brideau RJ, Homa FL, Hopkins TA, Knechtel ML, Oien NL, Pitts TW, Poorman RA, Wathen MW, Wieber JL.
J Med Chem (2005) 48 : 5794 -5804
PubMed 16134946 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.00 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL363625 2 7.16
CHEMBL194433 2 6.43
CHEMBL195453 2 6.34
CHEMBL29711 APHIDICOLIN 2 6.22
CHEMBL440033 2 6.11
CHEMBL193952 ZIDOVUDINE TRIPHOSPHATE -1.0 2 5.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL363625 IC50 = 70.0 nM 7.16
CHEMBL194433 IC50 = 370.0 nM 6.43
CHEMBL195453 IC50 = 460.0 nM 6.34
CHEMBL29711 APHIDICOLIN IC50 = 600.0 nM 6.22
CHEMBL440033 IC50 = 770.0 nM 6.11
CHEMBL29711 APHIDICOLIN IC50 = 2600.0 nM 5.58
CHEMBL193952 ZIDOVUDINE TRIPHOSPHATE IC50 = 5800.0 nM 5.24
CHEMBL193952 ZIDOVUDINE TRIPHOSPHATE IC50 = 12000.0 nM 4.92
CHEMBL195453 IC50 - nM -
CHEMBL440033 IC50 > 40000.0 nM -
CHEMBL363625 IC50 > 40000.0 nM -
CHEMBL194433 IC50 > 40000.0 nM -