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Assay Detail

CHEMBL873248

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of sodium dependent recovery of pH following imposed acidosis in AP1 cell line expressing the human NHE-1 isoform.
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type AP1
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Sodium/hydrogen exchanger 1 (CHEMBL2781)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Aminoimidazoles as bioisosteres of acylguanidines: novel, potent, selective and orally bioavailable inhibitors of the sodium hydrogen exchanger isoform-1.
Ahmad S, Ngu K, Combs DW, Wu SC, Weinstein DS, Liu W, Chen BC, Chandrasena G, Dorso CR, Kirby M, Atwal KS.
Bioorg Med Chem Lett (2004) 14 : 177 -180
PubMed 14684323 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.18 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL51363 1 8.22
CHEMBL51950 1 8.10
CHEMBL51879 1 8.05
CHEMBL297962 1 7.68
CHEMBL52381 1 7.41
CHEMBL49235 1 6.75
CHEMBL51727 1 6.12
CHEMBL301286 1 6.02
CHEMBL299011 1 5.58
CHEMBL50529 1 5.44
CHEMBL301068 1 5.42
CHEMBL52345 1 5.40
CHEMBL299962 1 5.31
CHEMBL50671 1 5.10
CHEMBL51010 1 5.00
CHEMBL50955 1 4.96
CHEMBL50670 1 4.52
CHEMBL299442 1 -
CHEMBL301321 1 -
CHEMBL299212 1 -
CHEMBL298358 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL51363 IC50 = 6.0 nM 8.22
CHEMBL51950 IC50 = 8.0 nM 8.10
CHEMBL51879 IC50 = 9.0 nM 8.05
CHEMBL297962 IC50 = 21.0 nM 7.68
CHEMBL52381 IC50 = 39.0 nM 7.41
CHEMBL49235 IC50 = 180.0 nM 6.75
CHEMBL51727 IC50 = 750.0 nM 6.12
CHEMBL301286 IC50 = 950.0 nM 6.02
CHEMBL299011 IC50 = 2600.0 nM 5.58
CHEMBL50529 IC50 = 3600.0 nM 5.44
CHEMBL301068 IC50 = 3800.0 nM 5.42
CHEMBL52345 IC50 = 4000.0 nM 5.40
CHEMBL299962 IC50 = 4850.0 nM 5.31
CHEMBL50671 IC50 = 8000.0 nM 5.10
CHEMBL51010 IC50 = 10000.0 nM 5.00
CHEMBL50955 IC50 = 11000.0 nM 4.96
CHEMBL50670 IC50 = 30000.0 nM 4.52
CHEMBL299442 IC50 > 30000.0 nM -
CHEMBL301321 IC50 > 30000.0 nM -
CHEMBL299212 IC50 > 30000.0 nM -
CHEMBL298358 IC50 > 30000.0 nM -