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Assay Detail

CHEMBL877707

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Concentration of compound required to induce 50 % of the maximum stimulation of alkaline phosphatase activity in Ishikawa cells
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Ishikawa
Confidence 1 — Organism
Curated By Intermediate

Target

Ishikawa (CHEMBL614649)
Type CELL-LINE
Organism Homo sapiens

Publication

3-[4-(1,2-Diphenylbut-1-enyl)phenyl]acrylic acid: a non-steroidal estrogen with functional selectivity for bone over uterus in rats.
Willson TM, Henke BR, Momtahen TM, Charifson PS, Batchelor KW, Lubahn DB, Moore LB, Oliver BB, Sauls HR, Triantafillou JA.
J Med Chem (1994) 37 : 1550 -1552
PubMed 8201587 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 8.12 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL135 ESTRADIOL 4.0 1 11.00
CHEMBL41069 1 8.64
CHEMBL38797 1 8.34
CHEMBL39416 1 8.16
CHEMBL36679 1 8.07
CHEMBL39227 1 7.96
CHEMBL288049 1 7.92
CHEMBL289612 1 7.77
CHEMBL38776 1 7.75
CHEMBL83 TAMOXIFEN 4.0 1 7.48
CHEMBL33899 1 7.24
CHEMBL36550 1 7.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL135 ESTRADIOL EC50 = 0.01 nM 11.00
CHEMBL41069 EC50 = 2.3 nM 8.64
CHEMBL38797 EC50 = 4.6 nM 8.34
CHEMBL39416 EC50 = 6.9 nM 8.16
CHEMBL36679 EC50 = 8.6 nM 8.07
CHEMBL39227 EC50 = 11.0 nM 7.96
CHEMBL288049 EC50 = 12.0 nM 7.92
CHEMBL289612 EC50 = 17.0 nM 7.77
CHEMBL38776 EC50 = 18.0 nM 7.75
CHEMBL83 TAMOXIFEN EC50 = 33.0 nM 7.48
CHEMBL33899 EC50 = 58.0 nM 7.24
CHEMBL36550 EC50 = 70.0 nM 7.16