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Assay Detail

CHEMBL880146

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Compound was tested for its potency at M-2 receptor by inhibiting forskolin induced c-AMP formation in CHO-M2 cells human M2 receptor
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type CHO-M2
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M2 (CHEMBL211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1-(1,2,5-Thiadiazol-4-yl)-4-azatricyclo[2.2.1.0(2,6)]heptanes as new potent muscarinic M1 agonists: structure-activity relationship for 3-aryl-2-propyn-1-yloxy and 3-aryl-2-propyn-1-ylthio derivatives.
Jeppesen L, Olesen PH, Hansen L, Sheardown MJ, Thomsen C, Rasmussen T, Jensen AF, Christensen MS, Rimvall K, Ward JS, Whitesitt C, Calligaro DO, Bymaster FP, Delapp NW, Felder CC, Shannon HE, Sauerberg P.
J Med Chem (1999) 42 : 1999 -2006
PubMed 10354408 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 6.17 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL60374 1 7.10
CHEMBL418491 1 7.08
CHEMBL291351 1 6.62
CHEMBL60479 1 6.28
CHEMBL303360 1 6.17
CHEMBL304247 1 6.17
CHEMBL60331 1 6.12
CHEMBL294082 1 6.10
CHEMBL417560 1 6.07
CHEMBL293982 1 6.04
CHEMBL60101 1 5.92
CHEMBL60777 1 5.83
CHEMBL64029 1 5.78
CHEMBL60833 1 5.64
CHEMBL291872 1 5.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL60374 EC50 = 80.0 nM 7.10
CHEMBL418491 EC50 = 83.0 nM 7.08
CHEMBL291351 EC50 = 241.0 nM 6.62
CHEMBL60479 EC50 = 528.0 nM 6.28
CHEMBL303360 EC50 = 673.0 nM 6.17
CHEMBL304247 EC50 = 683.0 nM 6.17
CHEMBL60331 EC50 = 763.0 nM 6.12
CHEMBL294082 EC50 = 791.0 nM 6.10
CHEMBL417560 EC50 = 853.0 nM 6.07
CHEMBL293982 EC50 = 904.0 nM 6.04
CHEMBL60101 EC50 = 1210.0 nM 5.92
CHEMBL60777 EC50 = 1480.0 nM 5.83
CHEMBL64029 EC50 = 1655.0 nM 5.78
CHEMBL60833 EC50 = 2280.0 nM 5.64
CHEMBL291872 EC50 = 2690.0 nM 5.57