Assay Detail
Binding
CHEMBL881383
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Transcriptional activation of estrogen receptor beta in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | U2OS |
| Confidence | 9 — Direct single protein target |
| Curated By | Expert |
Publication
Differential response of estrogen receptor subtypes to 1,3-diarylindene and 2,3-diarylindene ligands.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 8 | 8.17 | 10.01 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | 4.0 | 1 | 10.01 |
| CHEMBL306878 | — | — | 1 | 9.52 |
| CHEMBL69408 | — | — | 1 | 9.52 |
| CHEMBL371236 | — | — | 1 | 8.00 |
| CHEMBL199140 | — | — | 1 | 7.43 |
| CHEMBL370609 | — | — | 1 | 7.28 |
| CHEMBL48352 | — | — | 1 | 7.27 |
| CHEMBL371192 | — | — | 1 | 6.29 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | EC50 | = | 0.098 | nM | 10.01 |
| CHEMBL306878 | — | EC50 | = | 0.3 | nM | 9.52 |
| CHEMBL69408 | — | EC50 | = | 0.3 | nM | 9.52 |
| CHEMBL371236 | — | EC50 | = | 10.0 | nM | 8.00 |
| CHEMBL199140 | — | EC50 | = | 37.0 | nM | 7.43 |
| CHEMBL370609 | — | EC50 | = | 52.0 | nM | 7.28 |
| CHEMBL48352 | — | EC50 | = | 54.0 | nM | 7.27 |
| CHEMBL371192 | — | EC50 | = | 512.0 | nM | 6.29 |