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Assay Detail

CHEMBL887940

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of gamma secretase assessed as reduction of amyloid beta level in H4 cells
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type H4
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Gamma-secretase (CHEMBL2094135)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of (S)-2-((S)-2-(3,5-difluorophenyl)-2-hydroxyacetamido)-N-((S,Z)-3-methyl-4-oxo-4,5-dihydro-3H-benzo[d][1,2]diazepin-5-yl)propanamide (BMS-433796): a gamma-secretase inhibitor with Abeta lowering activity in a transgenic mouse model of Alzheimer's disease.
Prasad CV, Zheng M, Vig S, Bergstrom C, Smith DW, Gao Q, Yeola S, Polson CT, Corsa JA, Guss VL, Loo A, Wang J, Sleczka BG, Dangler C, Robertson BJ, Hendrick JP, Roberts SB, Barten DM.
Bioorg Med Chem Lett (2007) 17 : 4006 -4011
PubMed 17502137 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 8.18 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL392246 1 9.70
CHEMBL247361 1 9.52
CHEMBL397844 1 9.40
CHEMBL396808 1 9.30
CHEMBL247155 1 9.15
CHEMBL439210 1 9.00
CHEMBL429292 1 9.00
CHEMBL246785 1 8.89
CHEMBL397394 1 8.72
CHEMBL245385 1 8.70
CHEMBL397335 1 8.70
CHEMBL246575 1 8.52
CHEMBL246784 1 8.40
CHEMBL246369 1 8.22
CHEMBL397547 1 8.10
CHEMBL436997 1 8.05
CHEMBL247560 1 8.00
CHEMBL397548 1 8.00
CHEMBL247154 1 7.85
CHEMBL247360 1 7.85
CHEMBL396164 1 7.66
CHEMBL265897 1 7.62
CHEMBL396639 1 7.52
CHEMBL246573 1 7.37
CHEMBL397843 1 7.25
CHEMBL246370 1 7.23
CHEMBL246371 1 6.79
CHEMBL395198 1 6.57
CHEMBL393484 1 6.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL392246 IC50 = 0.2 nM 9.70
CHEMBL247361 IC50 = 0.3 nM 9.52
CHEMBL397844 IC50 = 0.4 nM 9.40
CHEMBL396808 IC50 = 0.5 nM 9.30
CHEMBL247155 IC50 = 0.7 nM 9.15
CHEMBL439210 IC50 = 1.0 nM 9.00
CHEMBL429292 IC50 = 1.0 nM 9.00
CHEMBL246785 IC50 = 1.3 nM 8.89
CHEMBL397394 IC50 = 1.9 nM 8.72
CHEMBL245385 IC50 = 2.0 nM 8.70
CHEMBL397335 IC50 = 2.0 nM 8.70
CHEMBL246575 IC50 = 3.0 nM 8.52
CHEMBL246784 IC50 = 4.0 nM 8.40
CHEMBL246369 IC50 = 6.0 nM 8.22
CHEMBL397547 IC50 = 8.0 nM 8.10
CHEMBL436997 IC50 = 9.0 nM 8.05
CHEMBL247560 IC50 = 10.0 nM 8.00
CHEMBL397548 IC50 = 10.0 nM 8.00
CHEMBL247154 IC50 = 14.0 nM 7.85
CHEMBL247360 IC50 = 14.0 nM 7.85
CHEMBL396164 IC50 = 22.0 nM 7.66
CHEMBL265897 IC50 = 24.0 nM 7.62
CHEMBL396639 IC50 = 30.0 nM 7.52
CHEMBL246573 IC50 = 43.0 nM 7.37
CHEMBL397843 IC50 = 56.0 nM 7.25
CHEMBL246370 IC50 = 59.0 nM 7.23
CHEMBL246371 IC50 = 162.0 nM 6.79
CHEMBL395198 IC50 = 269.0 nM 6.57
CHEMBL393484 IC50 = 750.0 nM 6.12