Assay Detail
Functional
CHEMBL888233
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at EP4 receptor expressed in HEK293 cells assessed as cAMP accumulation
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Target
Prostaglandin E2 receptor EP4 subtype (CHEMBL1836) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of novel prostaglandin analogs of PGE2 as potent and selective EP2 and EP4 receptor agonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 8 | 8.63 | 10.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL251505 | — | — | 1 | 10.00 |
| CHEMBL248679 | — | — | 1 | 9.30 |
| CHEMBL251709 | — | — | 1 | 8.70 |
| CHEMBL251710 | — | — | 1 | 8.52 |
| CHEMBL398947 | — | — | 1 | 8.40 |
| CHEMBL251504 | — | — | 1 | 7.82 |
| CHEMBL398948 | — | — | 1 | 7.68 |
| CHEMBL251294 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL251505 | — | EC50 | = | 0.1 | nM | 10.00 |
| CHEMBL248679 | — | EC50 | = | 0.5 | nM | 9.30 |
| CHEMBL251709 | — | EC50 | = | 2.0 | nM | 8.70 |
| CHEMBL251710 | — | EC50 | = | 3.0 | nM | 8.52 |
| CHEMBL398947 | — | EC50 | = | 4.0 | nM | 8.40 |
| CHEMBL251504 | — | EC50 | = | 15.0 | nM | 7.82 |
| CHEMBL398948 | — | EC50 | = | 21.0 | nM | 7.68 |
| CHEMBL251294 | — | EC50 | = | 0.002 | nM | - |