Assay Detail
Functional
CHEMBL888384
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Growth inhibition of methotrexate-resistant human CCRF-CEM R1 cells overexpressing DHFR
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | CCRF-CEM |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Design and synthesis of classical and nonclassical 6-arylthio-2,4-diamino-5-ethylpyrrolo[2,3-d]pyrimidines as antifolates.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 2 | 5.82 | 6.39 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 6.39 |
| CHEMBL438664 | — | — | 1 | 5.24 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | EC50 | = | 410.0 | nM | 6.39 |
| CHEMBL438664 | — | EC50 | = | 5700.0 | nM | 5.24 |