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Assay Detail

CHEMBL890112

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CCR5 expressed in CHO cells assessed as inhibition of MIP-1-alpha-stimulated calcium mobilization
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

C-C chemokine receptor type 5 (CHEMBL274)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Spirodiketopiperazine-based CCR5 antagonists: Lead optimization from biologically active metabolite.
Nishizawa R, Nishiyama T, Hisaichi K, Matsunaga N, Minamoto C, Habashita H, Takaoka Y, Toda M, Shibayama S, Tada H, Sagawa K, Fukushima D, Maeda K, Mitsuya H.
Bioorg Med Chem Lett (2007) 17 : 727 -731
PubMed 17118654 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.03 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL536743 1 7.55
CHEMBL559168 1 7.48
CHEMBL536754 1 7.28
CHEMBL535172 1 7.10
CHEMBL556995 1 7.08
CHEMBL537424 1 7.03
CHEMBL536298 1 6.89
CHEMBL558043 1 6.82
CHEMBL557789 1 6.68
CHEMBL536751 1 6.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL536743 IC50 = 28.0 nM 7.55
CHEMBL559168 IC50 = 33.0 nM 7.48
CHEMBL536754 IC50 = 53.0 nM 7.28
CHEMBL535172 IC50 = 79.0 nM 7.10
CHEMBL556995 IC50 = 84.0 nM 7.08
CHEMBL537424 IC50 = 94.0 nM 7.03
CHEMBL536298 IC50 = 130.0 nM 6.89
CHEMBL558043 IC50 = 150.0 nM 6.82
CHEMBL557789 IC50 = 210.0 nM 6.68
CHEMBL536751 IC50 = 400.0 nM 6.40