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Assay Detail

CHEMBL895599

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of MEK in mouse colon 26 carcinoma cells assessed as inhibition of ERK phosphorylation by ELISA
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Publication

4-anilino-5-carboxamido-2-pyridone derivatives as noncompetitive inhibitors of mitogen-activated protein kinase kinase.
Spicer JA, Rewcastle GW, Kaufman MD, Black SL, Plummer MS, Denny WA, Quin J, Shahripour AB, Barrett SD, Whitehead CE, Milbank JB, Ohren JF, Gowan RC, Omer C, Camp HS, Esmaeil N, Moore K, Sebolt-Leopold JS, Pryzbranowski S, Merriman RL, Ortwine DF, Warmus JS, Flamme CM, Pavlovsky AG, Tecle H.
J Med Chem (2007) 50 : 5090 -5102
PubMed 17880056 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.16 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL244488 1 8.70
CHEMBL442235 1 8.40
CHEMBL234887 1 8.30
CHEMBL243457 1 7.68
CHEMBL396548 1 7.52
CHEMBL105442 CI-1040 2.0 1 7.34
CHEMBL397811 1 7.20
CHEMBL387574 1 7.17
CHEMBL393283 1 7.03
CHEMBL234890 1 7.02
CHEMBL391975 1 6.96
CHEMBL235780 1 6.92
CHEMBL233627 1 6.82
CHEMBL395231 1 6.75
CHEMBL237257 1 6.37
CHEMBL396631 1 6.20
CHEMBL233626 1 5.41
CHEMBL234237 1 -
CHEMBL237469 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL244488 IC50 = 2.0 nM 8.70
CHEMBL442235 IC50 = 4.0 nM 8.40
CHEMBL234887 IC50 = 5.0 nM 8.30
CHEMBL243457 IC50 = 21.0 nM 7.68
CHEMBL396548 IC50 = 30.0 nM 7.52
CHEMBL105442 CI-1040 IC50 = 46.0 nM 7.34
CHEMBL397811 IC50 = 63.0 nM 7.20
CHEMBL387574 IC50 = 68.0 nM 7.17
CHEMBL393283 IC50 = 93.0 nM 7.03
CHEMBL234890 IC50 = 95.0 nM 7.02
CHEMBL391975 IC50 = 110.0 nM 6.96
CHEMBL235780 IC50 = 120.0 nM 6.92
CHEMBL233627 IC50 = 150.0 nM 6.82
CHEMBL395231 IC50 = 180.0 nM 6.75
CHEMBL237257 IC50 = 430.0 nM 6.37
CHEMBL396631 IC50 = 630.0 nM 6.20
CHEMBL233626 IC50 = 3900.0 nM 5.41
CHEMBL234237 IC50 > 5000.0 nM -
CHEMBL237469 IC50 > 5000.0 nM -