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Assay Detail

CHEMBL899661

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human androgen receptor expressed in HeLa cells assessed as inhibition of dihydrotestosterone induced transcriptional activity by reporter gene assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Androgen receptor (CHEMBL1871)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and structure-activity relationships of new steroidal compounds bearing a carboxy-terminal side chain as androgen receptor pure antagonists.
Tachibana K, Imaoka I, Yoshino H, Kato N, Nakamura M, Ohta M, Kawata H, Taniguchi K, Ishikura N, Nagamuta M, Onuma E, Sato H.
Bioorg Med Chem Lett (2007) 17 : 5573 -5576
PubMed 17804229 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.35 7.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL491 HYDROXYFLUTAMIDE 2.0 1 7.51
CHEMBL409 BICALUTAMIDE 4.0 1 6.85
CHEMBL235661 1 6.77
CHEMBL393320 1 6.72
CHEMBL238001 1 6.60
CHEMBL237999 1 6.52
CHEMBL235811 1 6.48
CHEMBL235445 1 6.38
CHEMBL235872 1 6.28
CHEMBL428021 1 6.18
CHEMBL235003 1 6.14
CHEMBL238000 1 6.07
CHEMBL393319 1 6.04
CHEMBL235871 1 6.04
CHEMBL391648 1 5.70
CHEMBL237971 1 5.24
CHEMBL236699 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL491 HYDROXYFLUTAMIDE IC50 = 31.0 nM 7.51
CHEMBL409 BICALUTAMIDE IC50 = 140.0 nM 6.85
CHEMBL235661 IC50 = 170.0 nM 6.77
CHEMBL393320 IC50 = 190.0 nM 6.72
CHEMBL238001 IC50 = 250.0 nM 6.60
CHEMBL237999 IC50 = 300.0 nM 6.52
CHEMBL235811 IC50 = 330.0 nM 6.48
CHEMBL235445 IC50 = 420.0 nM 6.38
CHEMBL235872 IC50 = 530.0 nM 6.28
CHEMBL428021 IC50 = 660.0 nM 6.18
CHEMBL235003 IC50 = 730.0 nM 6.14
CHEMBL238000 IC50 = 850.0 nM 6.07
CHEMBL393319 IC50 = 920.0 nM 6.04
CHEMBL235871 IC50 = 920.0 nM 6.04
CHEMBL391648 IC50 = 2000.0 nM 5.70
CHEMBL237971 IC50 = 5800.0 nM 5.24
CHEMBL236699 IC50 > 10000.0 nM -