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Assay Detail

CHEMBL900284

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]NDPMSH from human MC4R expressed in HEK293 cells
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Melanocortin receptor 4 (CHEMBL259)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, in vitro, and in vivo characterization of phenylpiperazines and pyridinylpiperazines as potent and selective antagonists of the melanocortin-4 receptor.
Tran JA, Jiang W, Tucci FC, Fleck BA, Wen J, Sai Y, Madan A, Chen TK, Markison S, Foster AC, Hoare SR, Marks D, Harman J, Chen CW, Arellano M, Marinkovic D, Bozigian H, Saunders J, Chen C.
J Med Chem (2007) 50 : 6356 -6366
PubMed 17994683 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 7.82 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL236521 1 9.22
CHEMBL393576 1 9.05
CHEMBL393584 1 8.60
CHEMBL266647 1 8.47
CHEMBL235556 1 8.43
CHEMBL236979 1 8.22
CHEMBL236731 1 8.01
CHEMBL377231 1 7.85
CHEMBL211564 1 7.72
CHEMBL213122 1 7.60
CHEMBL236978 1 7.44
CHEMBL236980 1 7.41
CHEMBL393583 1 7.36
CHEMBL213738 1 7.12
CHEMBL236802 1 6.58
CHEMBL236804 1 6.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL236521 Ki = 0.6 nM 9.22
CHEMBL393576 Ki = 0.9 nM 9.05
CHEMBL393584 Ki = 2.5 nM 8.60
CHEMBL266647 Ki = 3.4 nM 8.47
CHEMBL235556 Ki = 3.7 nM 8.43
CHEMBL236979 Ki = 6.0 nM 8.22
CHEMBL236731 Ki = 9.7 nM 8.01
CHEMBL377231 Ki = 14.0 nM 7.85
CHEMBL211564 Ki = 19.0 nM 7.72
CHEMBL213122 Ki = 25.0 nM 7.60
CHEMBL236978 Ki = 36.0 nM 7.44
CHEMBL236980 Ki = 39.0 nM 7.41
CHEMBL393583 Ki = 44.0 nM 7.36
CHEMBL213738 Ki = 75.0 nM 7.12
CHEMBL236802 Ki = 260.0 nM 6.58
CHEMBL236804 Ki = 890.0 nM 6.05