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Assay Detail

CHEMBL900876

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at rat histamine H3 receptor expressed in HEK239 cells assessed as inhibition of RAMH-stimulated [35S]GTP-gamma-S binding
14
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type HEK239
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Histamine H3 receptor (CHEMBL4124)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis, potency, and in vivo profiles of quinoline containing histamine H3 receptor inverse agonists.
Altenbach RJ, Liu H, Banfor PN, Browman KE, Fox GB, Fryer RM, Komater VA, Krueger KM, Marsh K, Miller TR, Pan JB, Pan L, Sun M, Thiffault C, Wetter J, Zhao C, Zhou D, Esbenshade TA, Hancock AA, Cowart MD.
J Med Chem (2007) 50 : 5439 -5448
PubMed 17918921 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
pKb 7 - -
Ki 7 8.60 9.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL392573 2 9.04
CHEMBL237394 2 9.00
CHEMBL237191 2 8.71
CHEMBL442242 2 8.51
CHEMBL263101 2 8.45
CHEMBL351231 2 8.34
CHEMBL393581 2 8.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL392573 Ki = 0.91 nM 9.04
CHEMBL237394 Ki = 1.0 nM 9.00
CHEMBL237191 Ki = 1.95 nM 8.71
CHEMBL442242 Ki = 3.09 nM 8.51
CHEMBL263101 Ki = 3.55 nM 8.45
CHEMBL351231 Ki = 4.57 nM 8.34
CHEMBL393581 Ki = 7.08 nM 8.15
CHEMBL351231 pKb = 8.34 -
CHEMBL263101 pKb = 8.45 -
CHEMBL442242 pKb = 8.51 -
CHEMBL392573 pKb = 9.04 -
CHEMBL237191 pKb = 8.71 -
CHEMBL393581 pKb = 8.15 -
CHEMBL237394 pKb = 9.0 -